Session Information
Date: Tuesday, September 24, 2019
Session Title: Parkinsonisms and Parkinson-Plus
Session Time: 1:45pm-3:15pm
Location: Agora 3 West, Level 3
Objective: The aim of this study is to evaluate the effect of Dichloromethane extract (DCMex) of Scutellaria Pinnatifida (S) on of alpha-synuclein (ASN) and effect of it against the toxicity induced by ASN aggregates and on SHSY5Y cell line.
Background: Parkinson’s disease (PD), is one of the prevalent and progressive nervous system disorder which affects nearly 1% of adults older than 60 years. Substantial evidence confirms that aggregation of alpha-synuclein (ASN) has a significant role in the pathogenesis of PD and other relative diseases [1,2]. is generally a natively unfolded protein in the cytoplasm, which possesses little or no ordered structure [3]. Under unknown conditions, the native structures of the ASN shift to conformations, prone to form amyloid fibrils which are toxic. Several studies have discovered natural compounds with the ability to prevent aggregation of ASN. A rich plant extensively used in traditional medicine is Scutellaria (Lamiaceae). One of the Iranian species of Scutellaria is S. [4]. Here we assess the effect of DCMex of S on of ASN and properties on SHSY5Y cell line against the toxicity of ASN aggregates and , a pesticide which can accumulate in the brain, impairs mitochondrial functions and finally leading to.
Method: The S was collected and the roots were extracted by Dichloromethane solvent. In order to evaluate the anti fibrillation effect of DCMex of SPinnatifida, ASN was co-incubated with and without DCMex in fibrillation condition, the rate of fibrillation was analyzed by fluorescence assay, red assay, AFM imaging, and microscopy imaging. Then, the of ASN incubated with and without DCMex on SHSY5Y cell lines was assessed by MTT, ROS and LDH tests. Finally, the protective effect of DCMex against toxicity induced by on SHSY5Y cell lines was analyzed by MTT and ROS.
Results: Our results show that DCMex of S inhibits fibrillation of alpha synuclein and also neutralized the toxicity of on SHSY5Y cell lines.
Conclusion: Collectively, our findings suggest that S may potentially be a valuable medicinal herb for the treatment of PD.
To cite this abstract in AMA style:
S. Parsafar, D. Morshedi, M. Mohammadi. DCM extract of Scutellaria Pinnatifida modulate alpha-synuclein fibrillation and protects dopaminergic SH-SY5Y cells against rotenone [abstract]. Mov Disord. 2019; 34 (suppl 2). https://www.mdsabstracts.org/abstract/dcm-extract-of-scutellaria-pinnatifida-modulate-alpha-synuclein-fibrillation-and-protects-dopaminergic-sh-sy5y-cells-against-rotenone/. Accessed June 15, 2025.« Back to 2019 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/dcm-extract-of-scutellaria-pinnatifida-modulate-alpha-synuclein-fibrillation-and-protects-dopaminergic-sh-sy5y-cells-against-rotenone/