Session Information
Date: Tuesday, June 21, 2016
Session Title: Genetics (NON-PD)
Session Time: 12:30pm-2:00pm
Location: Exhibit Hall located in Hall B, Level 2
Objective: Our objectives were to study the transcriptional expression of L1Rn elements in different brain regions and correlate with corresponding DNA methylation levels.
Methods: Real time PCR analysis using RNA isolated from various brain regions and various tissues from old and young wistar rats was carried out to determine the change in L1 transcripts. DNA methylation assay was performed using COBRA method (Yang et al, 2004).
Results: There was no significant change in the expression of L1Rn in various brain regions of 2 month old and 18 month old rats except cerebral cortex.
Conclusions: In conclusion, the degree of hypomethylation in promoter CpG islands in LINE-1 repetitive sequences do play essential role in LINE-1 element expression. Besides tissue specific factors do play pivotal role in LINE-1 expression.
To cite this abstract in AMA style:
S. Mukherjee, K. Upadhyaya, D. Sharma. Correlation among DNA methylation status and LINE-1 expression in rat brain [abstract]. Mov Disord. 2016; 31 (suppl 2). https://www.mdsabstracts.org/abstract/correlation-among-dna-methylation-status-and-line-1-expression-in-rat-brain/. Accessed May 9, 2025.« Back to 2016 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/correlation-among-dna-methylation-status-and-line-1-expression-in-rat-brain/