Objective: To evaluate the antiparkinsonian efficacy of silver nanoparticles synthesized from Eclipta prostrata (SEP) in a haloperidol-induced murine model of Parkinson’s disease (PD).

Background: Eclipta prostrata is a medicinal plant with established pharmacological properties. Green synthesis of silver nanoparticles enhances the bioavailability and central nervous system penetration of these neuroprotective phytochemicals.

Method: Parkinsonism was induced in male mice via haloperidol administration (1 mg/kg/day intraperitoneally for one week). Subjects were divided into six cohorts (n=12/group): a vehicle control, a haloperidol-only disease model, three experimental groups receiving oral SEP (50, 100, and 200 mg/kg/day) concurrently with haloperidol, and a positive control receiving levodopa (30 mg/kg). Motor and behavioral evaluations were conducted on days 1 and 8. Assessments included the hanging wire test for neuromuscular strength, the tardive dyskinesia test measuring vacuous chewing movements (VCMs), and the hole board test for exploratory behavior. Statistical analysis utilized one-way analysis of variance (ANOVA) followed by post-hoc Tukey testing.

Results: SEP administration at 100 and 200 mg/kg produced profound behavioral and biochemical improvements. Compared to the haloperidol-only group, SEP-treated mice demonstrated a significantly longer latency to fall in the hanging wire test (p<0.005), indicating rescued grip strength and motor coordination. Furthermore, SEP yielded a marked reduction in VCMs during tardive dyskinesia assessments (p<0.001). Exploratory behavior in the hole board test also significantly improved, evidenced by increased head dips and line crossings (p<0.001). Biochemically, SEP treatment robustly replenished the antioxidant defense status and significantly reduced tissue lipid peroxidation levels.

Conclusion: SEP exerts substantial antiparkinsonian and antioxidant effects in a haloperidol-induced murine model, significantly ameliorating motor deficits, dyskinesia, and oxidative stress. These findings highlight plant-derived silver nanoparticles as a highly promising candidate for PD management, warranting further mechanistic investigation to facilitate clinical translation.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/neuroprotective-and-antiparkinsonian-effects-of-green-synthesized-silver-nanoparticles-from-eclipta-prostrata-in-a-haloperidol-induced-murine-model/