Session Time: 1:45pm-3:15pm
Location: Hall 3FG
Objective: To evaluate the neuroprotective effect of Tinospora cordifolia aqueous extract (TCAE) in Parkinsonian mice.
Background: Parkinson’s disease, an age related neurodegenerative disorder, is characterized by progressive loss of dopaminergic neurons in substantia nigra pars compacta and projecting neurons terminals in striatum. Recently, several studies regarding Parkinson’s disease have proven the role of oxidative stress in neurodegeneration and neuroinflammation.
Methods: Twenty four Swiss albino mice were divided into three groups; Control, MPTP and MPTP+TCAE. Two doses of MPTP (30 mg/kg body wt.) were given intraperitoneally at 16 h interval. Normal saline was given to Control group whereas MPTP+TCAE treatment group received 200 mg/kg body wt. of TCAE orally for 21 days. After completion of treatment, mice brains were isolated for performing biochemical assays, western blotting, and Immunohistochemical analysis.
Results: From the Immunohistochemistry and western blot analysis, it is evident that TCAE inhibits the MPTP-induced activation of NF-κB and its associated pro-inflammatory cytokines. Through, Real-time PCR analysis it was revealed that pro-inflammatory cytokines were found to be up-regulated in MPTP intoxicated mice while TCAE treatment significantly restored their levels. In addition, the expression level of IL-10 was found to be decreased in the diseased condition which was further restored by TCAE treatment. Tyrosine hydroxylase, an important enzyme which is used as marker in Parkinson’s disease, its expression was found to be reduced in MPTP mice while on TCAE administration, its level was significantly restored.
Conclusions: Our result clearly indicates that Tinospora cordifolia provides neuroprotection against MPTP induced nigrostriatal dopaminergic neurodegeneration and shows potent anti-inflammatory activity.
To cite this abstract in AMA style:S. Singh, H. Birla, S. Rai, W. Zahra, S. Singh. Neuroprotective role of Tinospora cordifolia in MPTP induced Parkinsonian mouse model [abstract]. Mov Disord. 2018; 33 (suppl 2). https://www.mdsabstracts.org/abstract/neuroprotective-role-of-tinospora-cordifolia-in-mptp-induced-parkinsonian-mouse-model/. Accessed December 5, 2023.
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MDS Abstracts - https://www.mdsabstracts.org/abstract/neuroprotective-role-of-tinospora-cordifolia-in-mptp-induced-parkinsonian-mouse-model/