Objective: (1) Quercetin is well tolerated bioflavonoid used as supplement for various disorders but problem is its low oral bioavailability and (2) Piperine is combined to enhance bioavailability of quercetin used as neuromodulatory, neuroprotective in movement disorders like Parkinson’s disease

Background: MPTP is a neurotoxin which cause destruction of dopaminergic neurons, produces Parkinson’s like manifestations both in human and animals.  Quercetin possesses good antioxidant and neuroprotective activity but major complication is its poor oral bioavailability. So to overcome this hindrance the present study was designed to investigate the effect of quercetin along with bioenhancer piperine against MPTP induced neurotoxicity in rats

Methods: Rats were administered MPTP (100 μg/1 μL bilaterally) for 3 days (i.e. 1^st, 4^th and 7^th). Quercetin (25 and 50 mg/kg) and combination of quercetin (25 mg/kg) with piperine (2.5 mg/kg) was administered daily for 21 days starting from the 7^th day of 1^st MPTP injection. Body weight and behavioral observations (locomotor, Rotarod, Grip Strength and Narrow beam walk performance) were recorded at weekly intervals after MPTP treatment. On the 22^nd day, the animals were sacrificed and the rat striatum was isolated for the estimation of biochemical parameters (lipid peroxidation, glutathione and nitrite), determination of pro-inflammatory cytokine levels (TNF-α, IL-6 and IL-1b) and neurochemical analysis (Norepinephrine, 5-HT, GABA, glutamate, dopamine)

Results: The present finding had showed that chronic quercetin treatment for the 14 days significantly ameliorated the MPTP induced motor deficit, biochemical and neurochemical alterations in rats. Moreover combination of piperine (2.5 mg/kg) with quercetin (25 mg/kg) significantly potentiates the protective effect as compared to curcumin alone treated group

Conclusions: In conclusion the administration of combination of quercetin and piperine had significantly prevented the MPTP induced behavioral, biochemical and neurological alteration by enhancing antioxidant and anti-inflammatory properties in rats

References: Kumar, P., Kalonia, H and Kumar, A. (2011). “Role of LOX/COX pathways in 3‐nitropropionic acid‐induced Huntington’s Disease‐like symptoms in rats: protective effect of licofelone.” British journal of pharmacology. 164(2b), 644-654. Patel, AB., de Graaf, RA., Mason, GF., Rothman, DL., Shulman, RG., Behar KL (2005). The contribution of GABA to glutamate/glutamine cycling and energy metabolism in the rat cortex in vivo. Proc Natl Acad Sci USA. 102, 5588–93

« Back to 2017 International Congress

MDS Abstracts - https://www.mdsabstracts.org/abstract/piperine-potentiate-the-neuroprotective-effect-of-quercetin-against-mptp-induced-neurotoxicity-in-rats/