Objective: Objectives: to create and validate an MPTP neurotoxin-based zebrafish model of Parkinson’s disease and explore the therapeutic potential and neuroprotective effects of thymoquinone and edible bird’s nest in this model.
Background: PD is one of the most prevalent neurodegenerative illnesses. Due to its anti-inflammatory and antioxidant characteristics, Thymoquinone (TQ), and Edible Bird Nest (EBN) have been the subject of extensive research in a variety of disorders
Method: three-month-old zebrafish will receive an intraperitoneal injection of MPTP (100 g/g body weight) to create a PD model. Ten groups (5 M and 5 F) of adult Danio rerio zebra fish will be employed in this investigation, including: TQ in various dosages, TQ nanoparticles, vehicle, deprenyl medication, no therapy, EBN with various doses and a group with no induction of illness. After one hour, we will have confirmed Parkinson’s disease by gene expression analyses of Lrrk7, Park2, Park7, and SNCGB. Analysis of gene expression and behavioral investigations will be used to determine the effectiveness of the therapy 30 minutes after administration
Results: The tests are still being conducted. We anticipate that a reduced swimming distance, velocity, and mobility, as well as more freezing episodes, in the MPTP-induced group compared to the control group. Meanwhile, those in the vehicle-injected group should be able to continue swimming as usual. In addition, we anticipate that TQ and EBN will provide neuroprotection against MPTP effects, resulting in a ZF swimming pattern that is very similar to that of healthy individuals.
Conclusion: The ability to model PD in zebrafish has opened new avenues for research into the disease’s origins and progression. In this research, we will examine the potential neuroprotective benefits of TQ and EBN in the prevention of PD. The findings will be useful for developing an effective human therapy strategy against PD.
To cite this abstract in AMA style:W. Mohamed. Thymoquinone and Edible Bird Nest: The Secret Ingredients to Protect Against MPTP-induced zebrafish Parkinson’s disease [abstract]. Mov Disord. 2023; 38 (suppl 1). https://www.mdsabstracts.org/abstract/thymoquinone-and-edible-bird-nest-the-secret-ingredients-to-protect-against-mptp-induced-zebrafish-parkinsons-disease/. Accessed March 4, 2024.
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MDS Abstracts - https://www.mdsabstracts.org/abstract/thymoquinone-and-edible-bird-nest-the-secret-ingredients-to-protect-against-mptp-induced-zebrafish-parkinsons-disease/