Session Time: 1:45pm-3:15pm
Location: Exhibit Hall C
Objective: To identify the specificity of dopamine D2 receptor mediated signaling in cadmium mediated motor alterations
Background: Cadmium, a heavy metal, is to known to exert the toxicity in human body because of its wild industrial and anthropogenic applications. Human exposure to cadmium may therefore occur both in occupational and non-occupational settings and poses a serious risk to health and associated problems. Epidemiological and experimental studies carried out also suggest the role of cadmium in the progression of neurodegenerative diseases like Alzheimer’s and Parkinson’s. The studies carried out mechanism and the targets associated with this have not clearly been understood. The present study has therefore been aimed to investigate the mechanism of cadmium induced motor dysfunction.
Methods: Male Wistar rats were exposed to cadmium at a dose of 5.0 mg/kg, bw, p.o. for 28 days. Motor assessment in rats was done by using open field activity test, Rota rod and grip strength test. The other parameters were assessed by using the specific western blotting, RTpcr and IHC studies the specific radioligand technique was used to asses the integrity of Dopamine D2 receptors. Further, computational studies were also carried out using the auto dock4.2.
Results: A decrease in the motor activity and motor coordination was significantly observed in rats exposed to cadmium. The transcriptional and translational changes in DA-D2 receptors were found to be decreased with no changes in DA-D1 type receptors in cadmium exposed rats. Further the specific radioligand technique carries out using the Spiperone also confirm the finding of involvement of DA-D2 receptor in cadmium mediated motor alterations. A change in the DARPP32/PP1α also found to target the CREB which are found to be associated with functional changes.
Conclusions: The results of the present study significantly inhibit the involvement of DA-D2 type receptors mediated signaling in regulating the Parkinson like motor dysfunctions in rats.
To cite this abstract in AMA style:R. Gupta, R. Shukla, V. Khanna. D2 receptor mediated DARPP32/PP1α signaling is responsible for cadmium induced Parkinson’s like behavioral alterations in rats [abstract]. Mov Disord. 2017; 32 (suppl 2). https://www.mdsabstracts.org/abstract/d2-receptor-mediated-darpp32pp1%ce%b1-signaling-is-responsible-for-cadmium-induced-parkinsons-like-behavioral-alterations-in-rats/. Accessed December 11, 2023.
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MDS Abstracts - https://www.mdsabstracts.org/abstract/d2-receptor-mediated-darpp32pp1%ce%b1-signaling-is-responsible-for-cadmium-induced-parkinsons-like-behavioral-alterations-in-rats/