Objective: This study is aimed at evaluating the potential association between the single-nucleotide polymorphism of two functional clock genes (CLOCK gene rs1801260 and PER2 gene rs2304672) and susceptibility to Parkinson’s disease in people of Northeast China.

Background: The clock genes play an important role in in the pathophysiology of aging and oxidative stress injury of neurons. Recently, the clock genes that regulate circadian rhythm arouse many researchers’ interest, since a growing body of evidence suggests alterations of the circadian system may participate in the pathogenesis of PD.

Methods: 646 cases of PD (Male 316, Female 338) from consecutive outpatient and inpatient ward of our hospital were included in this study.  The technique of Kompetitive Allele Specific PCR (KASP) was applied to determine the frequency distribution of genotype and allele gene of CLOCK gene（CLOCK rs1801260，PER2 rs2304672）in both groups. After amplification, PCR plates were read with a Spectramax M5 FRET capable plate reader (Molecular Devices, Sunnyvale, CA, USA) using the recommended excitation and emission values. Data was then analyzed using SNPViewer software to identify SNP genotypes.

Results: The significant association was observed for CLOCK rs1801260 in a dominant model. The frequency of TC genotype, CC+TC genotype and C allele in PD group is significantly higher than in control group of allele gene of CLOCK rs1801260 (P<0.01, P =0.001, P =0.001, respectively). As for PER2, there was no significant difference in the distributions of genotypes and alleles of rs2304672 between PD patients and controls (P>0.05, P>0.05, respectively). The correlation still remained after adjusting for confounding risk factors of PD (P =0.034).

Conclusions: Polymorphism of CLOCK rs1801260 may increase susceptibility to Parkinson’s disease independent of other common risk factors in northeastern Chinese population. PER2 rs2304672 variants may have no association with susceptibility to Parkinson’s disease in northeastern Chinese.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/clock-rs1801260-polymorphism-is-associated-with-suscepibilty-of-parkinsons-disease-in-chinese-population/