Session Information
Date: Monday, October 8, 2018
Session Title: Parkinson's Disease: Non-Motor Symptoms
Session Time: 1:15pm-2:45pm
Location: Hall 3FG
Objective: To investigate the role of blockade D1-like and D2-like dopamine receptors within nucleus accumbens attenuate hyperalgesia responses in a 6-OHDA induced parkinson’s disease rat model.
Background: Pain is one of the most common non-motor symptoms in patients with Parkinson’s disease (PD). It can occur before motor symptoms and is also one of the major causes of misdiagnosis of early PD. But the underlying mechanisms of pain in Parkinson’s disease remain elusive.
Methods: Rotarod test and open field test were used to evaluate motor function. The von Frey test and radiant heat test were conducted to evaluate mechanical and thermal pain thresholds, respectively. Western blotting was used to evaluate the D1-like and D2-like dopamine receptors expression in the NAc. Immunohistochemistry was used to examine dopamine D1 receptor and D2 receptor NAc. HPLC was used to determine DA and DOPAC levels in NAc. D1 receptor and D2 receptor agonist or antagonist were microinjected into the NAc while their controls just received intraaccumbal saline at 0.5ul, respectively.
Results: The time on the rod was significantly reduced in 6-OHDA-treated rats in rotarod test. Both of mechanical and thremal nociceptive thresholds were reduced compared to saline-treated rats. The expression of the DA D1R and DA D2R were increased while DA D3R were no changes. DA concentration was reduced in the NAc. Furthermore, the administration of D1 receptor and D2 receptor antagonis (SCH-23390 and raclopride) significantly attenuated pain hypersensitivity, but the administration of agonist (SKF-38393 or quinpirole) or the selective dopamine reuptake inhibitor GBR-12090 had no effect on the pain hypersensitivity.
Conclusions: Our data suggested that the decreased DA contents, D1-like receptors and D2-like receptors within the NAc may be involved in hyperalgesia in the 6-OHDA-induced model of PD.
To cite this abstract in AMA style:
L. Li, CJ. Mao, XQ. Zhang, F. Wang, CF. Liu. Blockade D1-like and D2-like dopamine receptors within nucleus accumbens attenuate hyperalgesia responses in a 6-OHDA induced parkinson’s disease rat model [abstract]. Mov Disord. 2018; 33 (suppl 2). https://www.mdsabstracts.org/abstract/blockade-d1-like-and-d2-like-dopamine-receptors-within-nucleus-accumbens-attenuate-hyperalgesia-responses-in-a-6-ohda-induced-parkinsons-disease-rat-model/. Accessed June 15, 2025.« Back to 2018 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/blockade-d1-like-and-d2-like-dopamine-receptors-within-nucleus-accumbens-attenuate-hyperalgesia-responses-in-a-6-ohda-induced-parkinsons-disease-rat-model/