Session Information
Date: Tuesday, June 21, 2016
Session Title: Drug-induced movement disorders
Session Time: 12:30pm-2:00pm
Location: Exhibit Hall located in Hall B, Level 2
Objective: To determine the histochemical evaluation of the effect of Vitamin E on Cyanide – induced damage on the Prefrontal cortex of Sprague Dawley Rats.
Background: Cyanide is ubiquitous and the major source of human exposure is in diet. The exposure from diet is usually associated with consumption of cyanophoric plants such as cassava. Vitamin E is an important antioxidant. It acts as a free radical scavenger to prevent the by-products of chemical-cell interaction which causes cell damage.
Methods: Twenty male Sprague Dawley rats about 140-160 g were used for the study. Ethical approval was obtained from the University’s ethical committee. The rats were randomly divided into 4 groups of 5 rats each. The timeline of administration and treatment are as follow: Rats in group A= free access to normal saline. Rats in group B= treated with 10.50 mg/kg of potassium cyanide. Rats in group C= treated with 200 mg/kg of Vit. E. Rats in group D= were co-treated with 10.50 mg/kg of KCN and 200 mg/kg of Vit. E. The duration of treatment was 30d. 24hrs after the last administration, the rats were sacrificed by cervical dislocation: the fraction of the brain for tissue histochemistry was fixed in formol calcium and later processed for Nissl’s staining techniques (cresyl fast violet) and general neuronal outline (H&E). and the other fraction meant for enzyme and/or marker histochemistry was processed accordingly for the activities of Catalase, SOD, GSH, and GPX.
Results: Histology of the prefrontal cortex and biochemical assay of Catalase, SOD and GHS was carried out and the treated were compared with the normal and efficacy of Vitamin E was further potentiated as a free radical scavenger.
Conclusions: Vitamin E ameliorates the effects of cyanide on the PFC of rats. The maker of oxidative stress was statistically reduced in the rats in group D compared with the rats in group B. The histological profile of the PFC of rats in group A and C were preserved while that of the rats in group B displayed distorted cytoarchitectural profile with marked increase in apoptotic bodies, lateral deviation of neurons and marked increase in the activities of oxidative markers. The histoarchitecture of the rats in group D was also preserved. Neurobehavioural test on the motor function was done using rotariod and bar test.
To cite this abstract in AMA style:
A.O. Adekeye, P.D. Shallie. Histochemical evaluation of the effect of vitamin E on cyanide–induced damage on the prefrontal cortex of Sprague Dawley rats [abstract]. Mov Disord. 2016; 31 (suppl 2). https://www.mdsabstracts.org/abstract/histochemical-evaluation-of-the-effect-of-vitamin-e-on-cyanide-induced-damage-on-the-prefrontal-cortex-of-sprague-dawley-rats/. Accessed June 14, 2025.« Back to 2016 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/histochemical-evaluation-of-the-effect-of-vitamin-e-on-cyanide-induced-damage-on-the-prefrontal-cortex-of-sprague-dawley-rats/