Session Information
Date: Monday, June 20, 2016
Session Title: Surgical therapy: Parkinson's disease
Session Time: 12:30pm-2:00pm
Location: Exhibit Hall located in Hall B, Level 2
Objective: To develop a new dedicated stimulator, adapted for chronic experiments on rodent models and that mimics the conditions of DBS human therapy: bilateral, current-controlled biphasic stimulation and large range of frequencies, pulse widths and current intensities. Furthermore, dedicated software has been developed to program the stimulation parameters.
Background: High Frequency Stimulation (HFS) of the subthalamic nucleus (STN) is considered as a gold standard therapy for the alleviation of Parkinson’s disease motor symptoms when the patients develop motor fluctuations and dyskinesia in response to medication. However, although the beneficial effects of STN-HFS are well established, the underlying mechanisms are still under debate. In that context, there is a need for further investigations in animal models of Parkinson’s disease, especially to study the cellular substrate of chronic, not only acute, effects of STN-HFS.
Methods: Adult male Sprague-Dawley rats received stereotaxic bilateral microinjection of either 6-hydroxydopamine (6-OHDA) or NaCl (0.09%). Three weeks after they received implantation of the stimulating electrodes and the portable miniaturized stimulator.
Results: The stimulation device was well tolerated by the animals and during chronic stimulation, they had a good general welfare without any visible discomfort. STN-HFS (130 Hz frequency, 0.06mA pulse width) improved the motor deficits induced by 6-OHDA in rats in the open field as it significantly increased the number of spontaneous movements compared to the values obtained in the same animals without STN-HFS. Furthermore, it restored motor coordination by significantly increasing the time spent on the rotarod bar.
Conclusions: We successfully developed and validated a new implantable stimulator for freely moving rats, that can be used for different deep brain structures in different animal models of brain diseases.
To cite this abstract in AMA style:
F. Naudet, F. Kölbl, G. N'kaoua, E. Faggiani, B. Ribot, S. Renaud, N. Lewis, A. Benazzouz. In vivo validation of a new wireless portable stimulator for chronic deep brain stimulation in freely moving rats [abstract]. Mov Disord. 2016; 31 (suppl 2). https://www.mdsabstracts.org/abstract/in-vivo-validation-of-a-new-wireless-portable-stimulator-for-chronic-deep-brain-stimulation-in-freely-moving-rats/. Accessed June 15, 2025.« Back to 2016 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/in-vivo-validation-of-a-new-wireless-portable-stimulator-for-chronic-deep-brain-stimulation-in-freely-moving-rats/