Objective: In this study, we investigated the selenotranscriptome (24 selenoproteins in total) in five brain regions (cerebellum, substantia nigra, cortex, pons and hippocampus) in a two-phase manner using a mouse model of progressive PD.

Background: Genes of selenoproteome have been increasingly implicated in various aspects of neurobiology and neurological disorders, but remain largely elusive in Parkinson’s disease (PD).

Methods: We measured the selenoproteins level by qPCR.

Results: These results showed a wide range of changes in selenotranscriptome in the PD brain in a manner depending on selenoproteins and brain regions. While Selv mRNA was not detectable and Dio1& 3 mRNA levels were not affected by PD, 1, 11 and 9 selenoproteins displayed patterns of increase only, decrease only, and mixed response by PD, respectively, in these brain regions. In particular, the mRNA expression of Gpx1-4 showed only a decreased trend in the PD brain. In substantia nigra, levels of 17 selenoprotein mRNAs were significantly decreased whereas no selenoprotein was up-regulated in the PD mice. In contrast, the majority of selenotranscriptome did not change and a few selenoprotein mRNAs that respond displayed a mixed pattern of up- and down-regulation in cerebellum, cortex, hippocampus, and/or pons of the PD mice. Gpx4, Sep15, Selm, Sepw1, and Sepp1 mRNAs were most abundant across all these five brain regions.

Conclusions: Our results showed differential responses of selenoproteins to PD in various brain regions, providing critical selenotranscriptomic profiling for future functional investigation of individual selenoprotein in PD etiology.

« Back to 2016 International Congress

MDS Abstracts - https://www.mdsabstracts.org/abstract/selenotranscriptomic-analyses-identify-signature-selenoproteins-in-brain-regions-in-a-mouse-model-of-parkinsons-disease/