Objective: This study was designed with the objective to find out the possible synergistic effect of Curcumin (Cur) and Methanolic extract of Ginkgo biloba (EGB) Leaf in Parkinson’s disease (PD). Cur and EGB were explored for their neuroprotective effect in rotenone-induced neurodegeneration in mice model of Parkinson’s disease.

Background: Both Cur and EGB, are natural polyphenolic, highly potent bioactive compounds. Cur has been used for centuries in traditional medicines in India. Curcumin showed several biological and pharmacological activities including potent antioxidant, cardiovascular disease, anticancer, anti-inflammatory effects and neuroprotective effect. Ginkgo biloba is one of the most widely used herbal remedies in the world to treat the symptoms of early-stage Alzheimer’s disease, vascular dementia, peripheral claudication, and tinnitus of vascular origin.

Methods: Rotenone (1mg/kg/ i.p.) was used to induced PD in mice. Chronic rotenone administration for 21 days produced significant impairment in the behaviour pattern (learning, memory, motor coordination), decrease oxidative function (superoxide dismutase, catalase and reduced glutathione level) and mitochondrial function (Complex-I, Complex-II, Complex-III) as compared to normal control group of mice.

Results: Simultaneous treatment with Cur and EGB combination at different dose level (50, 100 and 200 mg/kg, p.o) for 21 days significantly improved behaviour parameters (P < 0.001), oxidative damage (P < 0.001) and mitochondrial enzyme complex activities (< 0.05, P < 0.01, P < 0.001) as compared to negative control (rotenone-treated) group. The results of present study showed that the Cur and EGB combination restored the motor deficit function and enhance oxidative and mitochondrial functions.

Conclusions: The outcome of present investigation confirms that the intraperitoneal administration of rotenone in mice induces neurobehavioral and biochemical changes mimicking those observed in Parkinson’s disease. Treatment of mice with combination of Cur and EGB produced significant neuroprotection probably mediated through its antioxidant activity and provides a strong justification for the therapeutic potential of these compounds in management of PD.

References: 1. Khatri D. K., Juvekar A. R, 2016. Neuroprotective effect of curcumin as evinced by abrogation of rotenone-induced motor deficits, oxidative and mitochondrial dysfunctions in mouse model of Parkinson’s disease. Pharmacology, Biochemistry and Behaviour, 150-151, 39-47. 2. Kim M. S. et al., 2004. Neuroprotective effect of Ginkgo biloba L. Extract in rat model of Parkinson’s disease. Phytotherapy Research, 18, 663-666.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/abrogation-of-rotenone-induced-behavior-biochemical-and-mitochondrial-deficits-in-mice-by-curcumin-and-ginkgo-biloba-new-combinational-therapy-approach-for-parkinsons-disease/