Session Information
Date: Tuesday, September 24, 2019
Session Title: Parkinsonisms and Parkinson-Plus
Session Time: 1:45pm-3:15pm
Location: Agora 3 West, Level 3
Objective: To examine alpha-synuclein expression in CD45+ and cytokine levels in blood of patients with dementia with Lewy bodies (DLB), Parkinson’s disease dementia (PDD).
Background: Lewy body dementias (LBD) are a group of diseases with cognitive decline (PDD, DLB) associated with abnormal deposits of alpha-synuclein in neurons that probably may activate neuroinflammation.
Method: 25 DLB patients, 19 PDD and 66 PD patients without dementia (PD) and 59 neurological healthy individuals (controls) as comparable groups were recruited for current study. CD45+ were selected by MACS system. Alpha-synuclein mRNA levels were estimated in CD45+ blood cells by quantitative RT-PCR with iTaq Universal SYBR Green Supermix (Bio-Rad, USA). Plasma cytokine levels (IFN-gamma, IL-2, IL-4, IL-6, IL-10, IL-12, IL-13, IL-21, IL-23, IL-1b, TNF-alpha, MCP-1) were detected by a Luminex array system.
Results: Alpha-synuclein expression in CD45+blood cells in DLB and PDD patients (1.408 (0.166-9.867), 2.132 (0.422-13.452), respectively) were significantly higher compared to PD patients 0.763 (0.004-12.853) (р=0.02, р=0.001, respectively) and controls 0.574 (0.013-7.497) (р=0.01, р=0.0003, respectively). Alpha-synuclein expression in CD45+blood cells in DLB and PDD patients did not show significant difference (p >0.05). TNF-alpha plasma levels were increased in DLB and PDD than in PD patients (p=0.025, p=0.033, respectively). IFN-gamma and IL-6 plasma levels were elevated in DLB patients compared to PD patients (p=0.0386, p=0.0328, respectively). Negative correlation of alpha-synuclein and IL-12 blood levels was shown in patients with dementia (DLB, PDD) (r=-0.43, p=0.01, respectively).
Conclusion: Our results showed that increased alpha-synuclein expression in CD45+ blood cells and increased proinflammatory cytokine plasma levels may be associated with the development of dementia in LBD. This study was supported by RFBR grant N18-315-00387 mol-a.
To cite this abstract in AMA style:
T. Usenko, M. Nikolaev, A. Bezroukova, A. Emelyanov, E. Gracheva, I. Milukhina, Y. Belceva, N. Zalutskaya, A. Timofeeva, S. Pchelina. Alpha-synuclein expression and cytokine profile in blood of patients with Lewy body dementias [abstract]. Mov Disord. 2019; 34 (suppl 2). https://www.mdsabstracts.org/abstract/alpha-synuclein-expression-and-cytokine-profile-in-blood-of-patients-with-lewy-body-dementias/. Accessed June 14, 2025.« Back to 2019 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/alpha-synuclein-expression-and-cytokine-profile-in-blood-of-patients-with-lewy-body-dementias/