Category: Parkinson's Disease: Genetics
Objective: The aim of this study was to establish candidate blood miRNAs biomarkers for PD by comparing differences in miRNAs expression between distinct PD subtypes.
Background: Blood circulating microRNAs (miRNAs) have been identified as promising biomarkers for Parkinson’s disease (PD). Over the last decade, several studies have reported deregulation of miRNAs expression in PD patients compared with healthy individuals; however, results of these biomarker studies have been inconsistent with a lack of reproducibility of candidate miRNAs across studies.
Method: Blood RNA was extracted from 13 treatment-naïve de novo PD patients without LRRK2 and GBA mutations, 30 idiopathic PD patients under dopamine replacement therapy (DRT) and without these mutations, 10 LRRK2-G2019S PD patients under DRT, 11 GBA PD patients under DRT, and 28 healthy controls (HC). MiRNAs profiling was performed using custom TaqMan Array cards. Differential expression analysis were performed between groups to examine differential miRNAs levels. The ability of the miRNAs to accurately discriminate PD subtypes was analyzed by receiver operating characteristic curves.
Results: The miRNA miR-1-3p significantly distinguished de novo PD from HC with an area under the curve of 77.9% (95% CI, 62.5-91.7). Besides, the area under the curve for the combination miR-1-3p/miR-195-5p was 91.9% (95% CI, 79.9-100.0) between LRRK2-G2019S PD and HC. Finally, miR-19b-3p/miR-223-3p showed an area under the curve of 89.7% (95% CI, 80.1–99.3) between GBA and idiopathic PD.
Conclusion: This study provides data supporting the potential of miR-1-3p to be used as an early diagnostic biomarker for PD. Six additional blood-based miRNAs (miR-181c-3p, miR-185-5p, miR-195-5p, miR-19b-3p, miR-223-3p, and miR-3613-3p) were reported as possible candidates related to the different PDsubtypes. Further studies would be necessary to fully validate the changes in these miRNAs in order to establish them as reliable biomarkers for PD.
To cite this abstract in AMA style:
MT. Periñán, P. Gómez-Garre, S. Jesús, D. Buiza-Rueda, MV. Jimenez-Jaraba, D. Macías-García, AD. Adarmes-Gómez, P. Mir. Blood-based circulating microRNAs for discriminating between Parkinson’s disease subtypes [abstract]. Mov Disord. 2021; 36 (suppl 1). https://www.mdsabstracts.org/abstract/blood-based-circulating-micrornas-for-discriminating-between-parkinsons-disease-subtypes/. Accessed June 15, 2025.« Back to MDS Virtual Congress 2021
MDS Abstracts - https://www.mdsabstracts.org/abstract/blood-based-circulating-micrornas-for-discriminating-between-parkinsons-disease-subtypes/