Objective: The study aims to identify the significant altered long non-coding RNAs (LncRNA) mRNAs expression profiles of blood leukocytes from Parkinson’s disease (PD) patients, which may contribute to find certain LncRNAs as potential diagnosis biomarkers for PD and provide crucial clues for the studies exploring the LncRNA mechanism in the pathogenesis of PD.

Background: Parkinson’s disease (PD) is a high incidence disabling degenerative disease with unclear pathogenesis. Despite intense investigation , the lacking of reliable biomarkers result that the diagnosis of PD have still relied on the experiences of clinical doctors in a remarkable degree since James Parkinson had reported it the first time in 1817. Researches have illuminated that peripheral immune inflammation play a vital role in the pathogenesis of PD. Long noncoding RNA (LncRNA) is involved in the regulation of gene expression at epigenetics, transcriptional and post-transcriptional levels.

Methods: Peripheral blood leucocytes specimens were obtained from 5 clinically established PD patients[1] and 5 age and sex matched healthy controls and LncRNAs and mRNAs expression profiles were detected by microarray simultaneously. In the purpose of verifying the reliability of microarray date, quantitative real-time PCR. (qRT-PCR) of 6 LncRNAs and mRNAs selected randomly was followed. Bioinformatics tools and databases were employed to predict the potential functions of LncRNAs.

Results: Compared with healthy controls, PD patients had 102 upregulated LncRNAs, 28 downregulated LncRNAs, 12 upregulated mRNAs, 37 downregulated mRNAs(fold change≥2,p<0.05). The results of qRT-PCR, HOTAIRM1, AC131056.3, HOTAIRM1_2.1 and lnc-MOK-6:1 were upregulated in PD patients, while NR4A3 and DEFA4 downregulated, were consistent with microarray data. Several GO terms/ KEGG pathway such as immune response, leukocyte mediated immunity, neuroactive ligand-receptor interaction were enriched in gene lists, suggesting a potential correlation with PD.

Conclusions: The study is the first time to shed light on the different leucocytes LncRNAs and mRNAs expression profiles simultaneously of PD patients from Asia. This information will promote further research on the diagnosis biomarkers and pathogenesis of PD.

References: 1. Postuma RB, Berg D, Stern M, et al. MDS clinical diagnostic criteria for Parkinson’s disease. Mov Disord 2015; 30(12): 1591-601.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/distinct-blood-leukocytes-expression-profiles-of-long-non-coding-rnas-and-mrnas-in-parkinsons-disease-patients/