Objective: To evaluat rotene model of parkinsonian syndrome in rats.

Background: Rotenone is of highly lipophilic substances that can freely enter hematoencephalic barrier through and biological membranes. The mechanism of action of rotenone as mechanism MPTP actions associated with mitochondrial respiratory chain. The advantage of the method rotenone modeling Parkinson’s disease is ability rotenone to provoke dopaminergic neurodegeneration, most similar in its symptoms and molecular biological characteristics to those of Parkinson’s disease.

Methods: In order to simulate Parkinson’s disease, rotenone is used primarily on cellular structures and embryological studies. In adult animals, primarily rats, rotenone used less frequently because of its low chemical stability in animal tissues and body fluids. rotenone administered by infusion, by micropumps, preferably intravenously.

Results: Behavior of animals studied at 3, 6 and 9 days of the experiment. Used a series of tests for the detection of extrapyramidal disorders, as well as test apomorphine verticalization. Apomorphine is administered at a dose of 1 mg / kg subcutaneously 5 min assess vertical activity, and after 10 min – postural instability. Also used unilateral introduction of rotenone (12 .mu.g in 0.5 .mu.l with Dimexidum a rate of 0.1 .mu.l / min.) in «medial forebrain bundle» by coordinates (AP: +0,2; L: ± 1,8; DV: 8 mm) stereotactic atlas.

Conclusions: Intranigralnoe single unilateral introduction of rotenone in small doses on today is available as one of the adequate models of parcinsonian syndrome of rats, which reproduces lengthy neurochemical and neuropathological changes similar to the changes in Parkinson diseases in the nigrostriatal system. Application of this method rotenone administration (doses of 3, 6 and 12 .mu.g) caused no change in peripheral organs for 4 weeks follow-up.

References: Winkler C., Kirik D., Bjorklund A., Cenci M.A. L-DOPA-induced dyskinesia in the intrastriatal 6-hydroxydopamine model of parkinson’s disease: relation to motor and cellular parameters of nigrostriatal function. Neurobiol Dis.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/experimental-rotenone-model/