Session Information
Date: Monday, September 23, 2019
Session Title: Clinical Trials, Pharmacology and Treatment
Session Time: 1:45pm-3:15pm
Location: Agora 3 West, Level 3
Objective: This study is aimed to investigate whether the number and the contents of serum exosomes can serve as a useful biomarker for the diagnosis of Parkinson’s disease (PD).
Background: Pathological features of PD include the formation of Lewy bodies/neurites of which the main component is α-synuclein (α-syn). Exosomes, nano-sized extracellular vesicles, play important roles in many physiological processes in the central nervous system, but also in the pathological processes of neurodegenerative disorders including PD. Exosomal α-syn secretion can attenuate intracellular a-syn burden whereas it may facilitate the propagation of α-syn.
Method: fifty-three PD patients who fulfilled the criteria of clinically established PD in the MDS-PD criteria and seventeen non-PD controls were recruited. Exosomes, prepared from serum by the conventional gradient centrifugation were enriched for multiple exosomal markers. The number of exosomes quantified by nanoparticle tracking system. The amounts of α-syn in total exosomal and media were quantified by ELISA. The correlation between exosomes and the heart to mediastinum (H/M) uptake ratio from cardiac-123I meta-iodobenzylguanidine (MIBG) was evaluated.
Results: While no significant difference was observed in the amount of α-syn either in exosomes and media between PD and non-PD patients, there was a tendency that the number of exosomes was lower in PD compared to non-PD patients (p=0.065). There was no significant correlation between the number; the contents of exosomes and early and delayed H/M ratios from MIBG.
Conclusion: Our results are consistent with our previous findings from cell culture experiments, where increased intracellular a-syn levels reduce secretion of exosomes and a-syn. Further studies are required to determine whether this reduction of exosomes is statistically significant. We also plan to evaluate the antibodies that detect specific aggregate forms of α-syn, and to analyze exosomes that are separated depending on the host organs.
To cite this abstract in AMA style:
Y. Ishiguro, T. Tsunemi, A. Yoroisaka, N. Hattori. The evaluation of exosomes as a biomarker of Parkinson’s disease [abstract]. Mov Disord. 2019; 34 (suppl 2). https://www.mdsabstracts.org/abstract/the-evaluation-of-exosomes-as-a-biomarker-of-parkinsons-disease/. Accessed June 14, 2025.« Back to 2019 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/the-evaluation-of-exosomes-as-a-biomarker-of-parkinsons-disease/