Objective: The aim of the present study is to establish a reproducible in-vitro protocol to induce the human MSCs derived from peripheral blood, after mobilization from bone marrow, to differentiate into DA neurons; as a potential cellular therapy for PD.

Background: Neurodegenerative diseases represent a great medical challenge with marked consequences on quality of life of the patients and their families. Parkinson’s disease (PD) constitutes a relatively common neurodegenerative disorder characterized by defect in dopaminergic neurons. The regenerative effect of mesenchymal stem cells (MSCs) stimulated research into their effect in treatment of PD.

Methods: Leukapharesis-derived MSC were isolated from 10 leukapharesis products using plastic adherence. Isolated MSCs were passaged, and passage III cells were induced to dopaminergic neurones using two-step protocol applying sequentially nerve growth factor and addition of ascorbic acid. Neuronal induction was evaluated by immunostaining against neurofilament protein, while dopaminergic neurons induction was evaluated using immunostaining against tyrosine hydroxylase.

Results: MSCs were isolated in a rate of 0.12%-0.15% of leukapharesis cells, with viability ranging from 70-96%. NF positivity was 15.38±3.84, while the percentage of cells stained for TH was 5.94±0.65.MSCs could be successfully induced to dopaminergic neuron-like cells in vitro.

Conclusions: This study suggests that MSCs might have a great therapeutic potential for neurological diseases since they could be easily obtained from peripheral blood after theirmobilization using G-CSF. In addition, they could be isolated,expanded and differentiated into DA neurons.

Yes, It is a published article at American Journal of Bioscience and Bioengineering. doi: 10.11648/j.bio.s.2015030401.12.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/two-step-induction-of-dopaminergic-neurons-differentiation-of-leukapharesis-derived-mesenchymal-stem-cells/