Session Information
Date: Monday, September 23, 2019
Session Title: Neuropharmacology
Session Time: 1:45pm-3:15pm
Location: Les Muses Terrace, Level 3
Objective: The effect of the D2-antagonist haloperidol on single unit activity (SU) and local field potentials (LFPs) of the cerebellar dentate nucleus and motor cortex (MCtx) and modulation of the entopeduncular nucleus (EPN) by deep brain stimulation (DBS) were studied in urethane anesthetized rats.
Background: The cortico basal ganglia circuitry plays a crucial role in the control of movement, sensorimotor integration, and also limbic and associative integrity. However, little is known about the interference with cerebellar nuclei. Here, we investigate the effect of dopamine blockade and basal ganglia neuromodulation on the neuronal activity of the dentate nucleus.
Method: We recorded dentate nucleus SU activity together with LFPs and motor cortex electrocorticogram (MCtx-ECoG) under urethane anesthesia (1.4 g/kg, i.p.) in rats before and after Haldol injection (1mg/kg). Thereafter, 30 sec EPN-DBS (130 Hz, 200 µA current, with 120 µs biphasic square wave pulses) was applied and the neuronal activity was re-assessed. For the LFPs, the signal was filtered between 0.1 and 140 Hz, SU signals were bandpass-filtered between 500 and 5000 Hz. The firing of each neuron was recoded for 10 to 12 min after signal stabilization and SU were sorted on the base of 3:1 signal to noise ratio.
Results: Injection of haloperidol decreased the SU activity in the dentate nucleus, while EPN DBS resulted in partial recovery of the firing activity. Further, the coefficient of variation of firing activity was increased with injection of Halo and EPN DBS. After injection of haloperidol the power of theta (4-8Hz) frequency decreased in the MCtx and increased in the dentate nucleus. In contrast, the power of beta (12-30Hz) was increased in MCtx and decreased in the dentate nucleus. EPN DBS compensated haloperidol-induced opposite effects of oscillatory activity in the MCtx and dentate nucleus.
Conclusion: Cerebro-cerebellar networks interact via the basal ganglia and neuromodulation of the EPN may alter cerebellar activity after dopamine blockade.
To cite this abstract in AMA style:
K. Schwabe, A. Abdulbaki, S. Helgers, J. Krauss, M. Alam. Effect of dopamine D2-receptor antagonist and basal ganglia neuromodulation on neuronal activity in the cerebral motor area and cerebellar dentate nucleus [abstract]. Mov Disord. 2019; 34 (suppl 2). https://www.mdsabstracts.org/abstract/effect-of-dopamine-d2-receptor-antagonist-and-basal-ganglia-neuromodulation-on-neuronal-activity-in-the-cerebral-motor-area-and-cerebellar-dentate-nucleus/. Accessed June 15, 2025.« Back to 2019 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/effect-of-dopamine-d2-receptor-antagonist-and-basal-ganglia-neuromodulation-on-neuronal-activity-in-the-cerebral-motor-area-and-cerebellar-dentate-nucleus/