Session Information
Date: Thursday, June 23, 2016
Session Title: Neuropharmacology
Session Time: 12:00pm-1:30pm
Location: Exhibit Hall located in Hall B, Level 2
Objective: The aim of the present study was to investigated using microdialysis methodology the impact of 1MeTIQ on the disturbances of in vivo dopamine release evoked by the neurotoxin, 6-OHDA.
Background: 6-OHDA is well known neurotoxin, which is used as animal model of Parkinson’s disease. 6-OHDA produces free radicals production and inhibits function of the mitochondrial complex I and IV. 1-Methyl-1,2,3,4-tetrahydroisoquinoline (1MeTIQ) is an endogenous compound, which exhibits the neuroprotective, antidepressant-like effect and MAO-inhibiting properties [Antkiewicz-Michaluk et al. 2011; Wasik et al. 2015].
Methods: 6-OHDA was given unilaterally into the right substantia nigra (8µg/20µl). 1MeTIQ was administered peripherally (i.p.) acute or chronic (during 14 days) in a dose 50 mg/kg and after 14 days the microdialysis study was performed. The dopamine and its metabolites were assayed in dialysates using HPLC with ED.
Results: The present study showed that unilaterally administration of 6-OHDA caused significant decrease the release of dopamine into the extracellular space of about 80% in the basal samples, continuing until the end of experiment. This effect of 6-OHDA was completely blocked by chronic treatment with an endogenous amine, 1MeTIQ.
Conclusions: 1MeTIQ has shown the neuroprotective activity in animal model of Parkinson’s disease. The mechanism liable for the neuroprotection of 1MeTIQ against 6-OHDA neurotoxicity may be more complex and especially seems to be connected with the antagonism to 6-OHDA-induced oxidative stress and sweeping up of free radicals production in the brain structures. Acknowledgements: The project was financed by the statutory founds of the Neurochemistry Department of the Institute of Pharmacology PAS, Kraków, Poland and by the grant No: 2012/07/B/NZ7/01149.
To cite this abstract in AMA style:
A. Wasik, I. Romanska, L. Antkiewicz-Michaluk. Neuroprotection effect of an endogenous amine, 1MeTIQ against disturbances in dopamine release induced by the neurotoxin, 6-OHDA: In vivo microdialysis study [abstract]. Mov Disord. 2016; 31 (suppl 2). https://www.mdsabstracts.org/abstract/neuroprotection-effect-of-an-endogenous-amine-1metiq-against-disturbances-in-dopamine-release-induced-by-the-neurotoxin-6-ohda-in-vivo-microdialysis-study/. Accessed June 15, 2025.« Back to 2016 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/neuroprotection-effect-of-an-endogenous-amine-1metiq-against-disturbances-in-dopamine-release-induced-by-the-neurotoxin-6-ohda-in-vivo-microdialysis-study/