Session Information
Date: Tuesday, June 21, 2016
Session Title: Therapy in movement disorders: Gene and cell-based therapies
Session Time: 12:30pm-2:00pm
Location: Exhibit Hall located in Hall B, Level 2
Objective: To investigate the effect of undifferentiated versus differentiated CD34 stem cells series on paraquat model of PD and to elucidate the mechanism of action of stem cells in ameliorating the degenerative changes induced by Paraquat.
Background: Parkinson’s disease (PD) is a neurodegenerative disorder caused by reduction in striatal dopamine due to progressive loss of dopaminergic neurons in the substantia nigra pars compacta. Paraquat (PQ) is a herbicide (1,1-dimethyl-4,4-bipyridium) with some chemical structure similarities to MPP+ (1-methyl-4-phenylpyridinium) and has been suggested as a possible PD inducing agent. Mesenchymal stem cells (MSCs) have the potential to differentiate into other types of tissue-forming cells e.g neurons that can be a potential therapy for PD.
Methods: MSCs were isolated from an aspirate of bone marrow; preparation protocol was mainly based on the frequent medium change in primary culture and diminishing the trypsinization time. Paraquat model was developed by injecting male albino rat two successive doses of interaperitoneal paraquat (Each one equal 7.5mg/kg/week). After developing the motor symptoms of PD, animals received IV injections with differentiated or undifferentiated cells (MSCs) 2000cell/1g, processed with growth factor for dopaminergic neuron. Scarification done after 9 weeks and brains used for histological and immunocytochemical studies.
Results: Stem cells improved paraquat induced motor deficits increased ambulation, and mobility duration in Parkinsonian mice. They also improved neuronal degenerative changes specially with using differentiated series.Undifferentiated stem cells increase nerve cell count significantly (P< 0.001) by (23%) with no significant increase in glial cell count (7%). Differentiated nerve cell counts increased significantly (P< 0.001) by (25%) and significant increase in glial cell counts by (22%) when compared with that of PQ.
Conclusions: Differentiated stem cells ameliorated motor, and histological deficits in Paraquat Parkinsonian rats, these effects were superior to those produced by undifferentiated.
To cite this abstract in AMA style:
S.I. El-Jaafary, H.S. Abdel Tawab, M.Y. Elwash, H. Gabr, H.M. Saad Eldien. Differentiated versus undifferentiated mesenchymal stem cell therapy in paraquat model of Parkinson’s disease [abstract]. Mov Disord. 2016; 31 (suppl 2). https://www.mdsabstracts.org/abstract/differentiated-versus-undifferentiated-mesenchymal-stem-cell-therapy-in-paraquat-model-of-parkinsons-disease/. Accessed June 15, 2025.« Back to 2016 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/differentiated-versus-undifferentiated-mesenchymal-stem-cell-therapy-in-paraquat-model-of-parkinsons-disease/