Objective: To explore the antioxidant and anti-inflammatory role of Carvacrol via Nrf2-NLRP3 axis in rotenone induced PD mice model
Background: NLRP3 inflammasome mediated neuroinflammation is one of the pathogenic mechanisms implicated in PD. Oxidative stress is the key factor which is involved in both priming and activation of NLRP3 inflammasome. Carvacrol is a known antioxidant and anti-inflammatory phytoconstituent which subsides neuroinflammation directly and indirectly.
Method: Swiss albino mice were treated with rotenone (1 mg/kg i.p.) with concomitant administration of carvacrol doses (25, 50 and 100 mg/kg p.o) for 21 days. Animals were assessed for Neurobehavioral (Baseline and end point) and molecular parameters. The brain samples were used for western blotting, ELISA and RT-PCR and immunofluorescence. Various inflammatory and antioxidant proteins were analyses using these techniques.
Results: The locomotor activity of the animals was significantly increased in the treatment group when compared with the disease group. Inflammatory proteins like NLRP3, ASC and inflammatory cytokines IL-1β, IL-6, THF-α and antioxidant proteins Nrf2, NQO-1, HO-1 were decreased in disease group of animals and increased respectively with treatment of carvacrol.
Conclusion: The present study reveals the neuroprotective role of carvacrol treatment to improve PD pathology in terms of improved motor activity and alleviated neuroinflammation via Nrf2-NLRP3 axis in rotenone induced PD mice model.
To cite this abstract in AMA style:D. Khatri, S. Shah, K. Tryphena. Evaluation of neuroprotective role of carvacrol via Nrf2-NLRP3 axis in rotenone induced PD mice model [abstract]. Mov Disord. 2023; 38 (suppl 1). https://www.mdsabstracts.org/abstract/evaluation-of-neuroprotective-role-of-carvacrol-via-nrf2-nlrp3-axis-in-rotenone-induced-pd-mice-model/. Accessed September 25, 2023.
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MDS Abstracts - https://www.mdsabstracts.org/abstract/evaluation-of-neuroprotective-role-of-carvacrol-via-nrf2-nlrp3-axis-in-rotenone-induced-pd-mice-model/