Session Information
Date: Monday, October 8, 2018
Session Title: Parkinson's Disease: Genetics
Session Time: 1:15pm-2:45pm
Location: Hall 3FG
Objective: Our study aims to perform detailed phenotyping of the A30P alpha-synuclein familial case of PD, allowing to identify underlying mechanisms of the disease that may translate into novel therapies.
Background: Parkinson’s disease (PD) is the second most common neurodegenerative disease. Approximately 20% of PD cases are known to be familial. From these, mutations in SNCA, the gene encoding alpha-synuclein, are linked to an autosomal dominant inheritance of the disease. In 1998, our group discovered the second known point mutation within the SNCA gene, causing an A30P exchange of the peptide sequence.
Methods: We generated first patient-derived cellular model of the A30P alpha-synuclein mutation carrier, by obtaining fibroblasts from an affected sibling of the index patient, an unaffected sibling of the patient, and an age-matched gender-matched non-PD control. We reprogrammed these fibroblasts into induced pluripotent stem cells (iPSCs), and differentiated them into midbrain dopaminergic neurons.
Results: We obtained enriched cultures of ≥90% midbrain neurons (FoxA2+/Tuj1+), with approximately 20% dopaminergic (TH+), for which we observed electrophysiological activity and dopamine release. We detected a significant reduction of the protein level of mitochondria complexes II, IV, and V in the patient lines compared with the controls, additionally we found a significant impairment of mitochondrial respiration and an increased susceptibility of the cells to oxidative stress. Gene edited isogenic controls were generated to dissect mutation-specific effects. Furthermore, we investigated mitochondria morphology and dynamics, and how these processes contribute to the dopaminergic neurodegeneration. Additionally, we are implementing previously established readouts on our high-throughput automated screening platform that will allow us to identify FDA approved compounds with potential to be re-purposed and used as PD treatment.
Conclusions: We believe that detailed phenotyping of the A30P alpha-synuclein monogenic case may help to identify underlying mechanisms of the disease that may translate into novel therapies, which would also apply to the more common sporadic forms of PD.
To cite this abstract in AMA style:
B. Santos, P. Barbuti, P. Antony, J. Arias, A. Hummel, J. Schwamborn, R. Krüger. Mitochondrial phenotype related to the A30P alpha-synuclein mutation as a patient-derived cellular model of Parkinson’s disease [abstract]. Mov Disord. 2018; 33 (suppl 2). https://www.mdsabstracts.org/abstract/mitochondrial-phenotype-related-to-the-a30p-alpha-synuclein-mutation-as-a-patient-derived-cellular-model-of-parkinsons-disease/. Accessed October 15, 2024.« Back to 2018 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/mitochondrial-phenotype-related-to-the-a30p-alpha-synuclein-mutation-as-a-patient-derived-cellular-model-of-parkinsons-disease/