Session Time: 1:45pm-3:15pm
Location: Hall 3FG
Objective: To test the hypothesis that Rose Anthocyanins, owing to their biological properties in vitro, protect against Parkinson’s pathophysiology in young Drosophila melanogaster.
Background: Anthocyanins are polyphenolic pigments found among colored fruits, vegetables and flowers. Vast classes of Rose Anthocyanins have been reported and possess antioxidant properties. Owing to their potential biological in vitro, and no data about the neuroprotective effects, it was envisaged to test their properties against a Parkinson’s phenotype in Drosophila.
Methods: Rose Anthocyanins (RA) were prepared using acidified water method from the red rose petals according to standard protocol, dried under pressure. Drosophila melanogaster larvae (2d old, 100 larvae/ group) maintained on a diet enriched with Rose anthocyanins (0.01-0.05% w/v in medium) were co-challenged with Parkinson toxins Rotenone and Paraquat (Rot-0.5mM). Larvae were monitored for behavioural manifestations and terminally for biochemical index.
Results: Larvae treated with RA demonstrated modulated redox status evident from increased thiol levels and reduced hydroperoxides levels in the homogenates. Further, there was induction of hsp70 levels among Bg9 larvae. Rot induced locomotor deficits in terms of number of line crossings on the grid in a concentration dependent manner. Interestingly, RA larvae performed better in an open field grid in terms of improved locomotor function as opposed to Rot groups. Biochemically dopamine levels were partially restored with Rose anthocyanins among Rot larvae. Further, RA reduced the Rot-induced oxidative stress among larvae in a concentration dependent manner.
Conclusions: Our data from drosophila are suggestive of potent neuro-attenuatory propensity of Rose anthocyanins against chemically-induced Parkinsonian phenotype among drosophila however studies are warranted for long term implications.
To cite this abstract in AMA style:G. Chandran, P. Chandrashekar, M. Jayaraju, J. Chauhan. Rose Anthocyanins protect against Parkinson’s pathophysiology: Behavioral and biochemical changes in young Drosophila [abstract]. Mov Disord. 2018; 33 (suppl 2). https://www.mdsabstracts.org/abstract/rose-anthocyanins-protect-against-parkinsons-pathophysiology-behavioral-and-biochemical-changes-in-young-drosophila/. Accessed November 29, 2023.
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MDS Abstracts - https://www.mdsabstracts.org/abstract/rose-anthocyanins-protect-against-parkinsons-pathophysiology-behavioral-and-biochemical-changes-in-young-drosophila/