Session Information
Date: Tuesday, June 21, 2016
Session Title: Parkinson's disease: Pathophysiology
Session Time: 12:30pm-2:00pm
Location: Exhibit Hall located in Hall B, Level 2
Objective: Both silent information regulator 1 (SIRT1) and hypoxia inducible factor 1 (HIF-1) have been found to play important roles in the pathophysiology of Parkinson’s disease (PD). However, their mechanisms and their relationship still require further study. In the present study, we focused on the change and relationship of SIRT1 and HIF-1α in PD.
Methods: PD cell models were established by using methyl-4-phenylpyridinium(MPP+), which induced inhibition of cell proliferation, cell cycle arrest and apoptosis.
Results: We found that the expression of HIF-1α increased and that SIRT1 expression was inhibited in MPP+ treated cells. With further analysis, we found that the acetylation of H3K14 combined with the HIF-1α promoter was dramatically increased in cells treated with MPP+, which resulted in the transcriptional activation of HIF-1α. Moreover, the acetylation of H3K14 and the expression of HIF-1α increased when SIRT1 was knocked down, suggesting that SIRT1 is involved in the epigenetic regulation of HIF-1α.
Conclusions: Our results support a link between PD and SIRT1/HIF-1α signaling, which may serve as a clue for understanding PD.
To cite this abstract in AMA style:
Y. Wu, S. Dong, Y. Guo, Y. Feng, X. Cui, S. Kuo, T. Liu. The epigenetic regulation of HIF-1α by SIRT1 in MPP+ treated SH-SY5Y cells [abstract]. Mov Disord. 2016; 31 (suppl 2). https://www.mdsabstracts.org/abstract/the-epigenetic-regulation-of-hif-1-by-sirt1-in-mpp-treated-sh-sy5y-cells/. Accessed December 11, 2024.« Back to 2016 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/the-epigenetic-regulation-of-hif-1-by-sirt1-in-mpp-treated-sh-sy5y-cells/