Session Time: 1:45pm-3:15pm
Location: Agora 2 West, Level 2
Objective: Present study aimed to investigate the changes in brain neurotransmitters and neuropetides in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse models of PD.
Background: Parkinson’s disease (PD), a neurotransmitter imbalance occurs in the extrapyramidal system with a dopamine and GABA deficiency and an acetylcholine and glutamate surplus. Neurodegeneration in PD occurs due to multiple pathways including oxidative stress, mitochondrial damage, protein aggregation. Neurotransmitters play a vital role in the functioning of brain.
Method: 65 animals of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse models for PD were studied. Determination of brain, classical neurotransmitters (dopamine, norepinephrine, acetylcholine, serotonin, gamma-aminobutyric acid (GABA), glutamate), adenosine and neuropeptides (cholecystokinin, dynorphin, neurotensin, substance P) in was carried out using high performance liquid chromatography HPLC) (1100 series, Agilent Technologies Inc., Santa Clara, CA, USA) with fluorescence detection was utilized to quantify metabolite concentrations. Standards were also run after every fourth sample as controls. Concentrations were corrected for potential metabolite loss during extraction using α-ABA as an internal standard.
Results: The mean values of brain norepinephrine, dopamine, glutamate, GABA and serotonin levels in MPTP rat models brain were significantly decreased compared to control group, which consequently, may changes motor and non-motor symptoms in PD mouse models. There was a significant alternations in adenosine and neuropeptides values in treatment group compared to control.
Conclusion: Brain neurotransmitters and neuropeptides play a vital role in brain functioning and also have important function in PD status. It remains to be examined the clinical efficacy of such neurotransmitters and to investigate in-depth the neural networks suggested in the extrapyramidal system.
To cite this abstract in AMA style:N. Kumar, R. Khanna. Brain neurotransmitters and neuropeptides alternations in Parkinson’s induced mouse models [abstract]. Mov Disord. 2019; 34 (suppl 2). https://www.mdsabstracts.org/abstract/brain-neurotransmitters-and-neuropeptides-alternations-in-parkinsons-induced-mouse-models/. Accessed November 29, 2023.
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MDS Abstracts - https://www.mdsabstracts.org/abstract/brain-neurotransmitters-and-neuropeptides-alternations-in-parkinsons-induced-mouse-models/