Session Time: 1:45pm-3:15pm
Location: Exhibit Hall C
Objective: We measured alpha-synuclein total (a-syntotal) and alpha-synuclein oligomers (a-synolig) concentration in 70 healthy subjects, of different ages (from 18 to 88 years old) to detect if salivary a-syn concentration is influenced by aging.
Background: The pathological hallmark of PD is a-syn deposition leading to the formation of Lewy bodies. A-syn can be detected in biological fluids including saliva. A-synolig exerts a crucial neurotoxic effect in PD. In PD patients we detected lower salivary a-syntotal and higher a-synolig than healthy subjects. Previous studies have shown that a-syn neuronal aggregates increase during aging.
Methods: Subjects have been divided into 5 groups of 14 subjects each one. The I group includes subjects from 18 to 26 years old (YO), the II group includes subjects from 30 to 36 YO, the III subjects from 40 to 46 YO, the IV subjects from 50 to 65 YO and the V includes subjects from 70 to 88 YO. Samples of saliva were collected following the protocol reported in previous study (Vivacqua et al., 2016). ELISA analysis was performed using two specific ELISA kits: SensoLyte 55550 for a-syntotal and MyBioSource MBS043824 for a-synolig. Statistical significance was evaluated by Mann-Whitney U test.
Results: No significative differences have been found in the concentration of a-syntot and a-synolig between the first four groups (p>0,05). The mean concentration of a-synolig is respectively: 0,10±0,081 ng/ml in the I group, 0,17±0,13 ng/ml in the II group, 0,19±0,16 ng/ml in the III group and 0,14±0,14 ng/ml in the IV group. Salivary a-syntotal was significantly lower, whereas a-synolig was significantly higher in the subjects of the V group confronting with the others (p<0,05). A-synolig concentration in the V group was 0,96±0,15 ng/ml. No significative correlations have been detected between a-syn concentration and age in the five groups.
Conclusions: In the subjects of the V group, decreased salivary concentration of a-syntotal may reflect the reduction of a-syn monomers (a-synmon), leading to the formation of insoluble intracellular inclusions during aging process. This is supported by the increased concentration of a-synolig in the same group. Moreover, the similar concentration of a-synolig in PD patients and in aging healthy subjects suggests that measurement of a-syn in saliva might be a useful method for help the diagnosis of PD especially in the early stages of the disease.
To cite this abstract in AMA style:G. Vivacqua, A. Fabbrini, A. Suppa, R. Mancinelli, G. Fabbrini, C. Colosimo, A. Berardelli. Salivary Alpha-Synuclein in Aging [abstract]. Mov Disord. 2017; 32 (suppl 2). https://www.mdsabstracts.org/abstract/salivary-alpha-synuclein-in-aging/. Accessed December 10, 2023.
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MDS Abstracts - https://www.mdsabstracts.org/abstract/salivary-alpha-synuclein-in-aging/