Session Information
Date: Tuesday, June 21, 2016
Session Title: Parkinson's disease: Genetics
Session Time: 12:30pm-2:00pm
Location: Exhibit Hall located in Hall B, Level 2
Objective: To investigate the neurotoxic role of overexpressing α-synuclein without Heat Shock Protein CNB115 (HSP115) in Parkinson’s disease Drosophila line.
Background: Parkinson’s disease (PD) is a common human neurodegenerative movement disorder and is characterized by a progressive loss of dopaminergic neurons and abnormal accumulation of α-synuclein (α-syn) into aggregates called Lewy bodies. On the other hand, HSP115 is a fly homologue of the mammalian serine protease, a mitochondrial protein that has 50% amino acid sequence identity, domain structure similar to human serine protease that is high homology to a bacterial survival factor, HtrA which protects bacterial cells from stress-induced toxicity due to misfolded proteins.
Methods: We Developed a Drosophila line expressing α-Syn without HSP115 by crossing α-Syn flies with knockout- HSP115 flies. Then α-Syn gene and knockout- HSP115 gene were identified after crossing with ELAV-GAL4 by polymerase chain reaction (PCR). We directed the pan neuronal and eye specific expression of α-syn without HSP115 by using ELAV-GAL4 and GMR-GAL4 driver line respectively.
Results: Abnormal accumulation of α-syn, reduced lifespan, locomotors dysfunction, dopaminergic neuronal loss and damage eyes were resulted in 30 days old in the developed α-syn without HSP115 Drosophila line that indicates a toxic dominant mechanism of α-syn for the etiopathogenesis of PD.
Conclusions: Our developed Drosophila line demonstrated the overexpression of intraneuronal accumulated α-syn protein without HSP115, suggesting the detrimental effects of α-syn without HSP115 in a Drosophila line of PD. References: 1. Amy M. Todd and Brian E. Staveley: Pink1 suppresses a-synuclein-induced phenotypes in a Drosophila model of Parkinson’s disease; Genome 51: 1040–1046 (2008). 2. Challa M, Malladi S, Pellock BJ, Dresnek D, Varadarajan S, Yin YW, White K, Bratton SB. Drosophila Omi, a mitochondrial-localized IAP antagonist and proapoptotic serine protease. EMBO J 2007; 26:3144-3156.
To cite this abstract in AMA style:
M.S. Islam, H.J. Kim, S.S. Hong. Reduced lifespan and climbing ability observed in the overexpressing human α-synuclein without heat shock protein CNB115 in Parkinson’s disease drosophila line [abstract]. Mov Disord. 2016; 31 (suppl 2). https://www.mdsabstracts.org/abstract/reduced-lifespan-and-climbing-ability-observed-in-the-overexpressing-human-synuclein-without-heat-shock-protein-cnb115-in-parkinsons-disease-drosophila-line/. Accessed May 18, 2025.« Back to 2016 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/reduced-lifespan-and-climbing-ability-observed-in-the-overexpressing-human-synuclein-without-heat-shock-protein-cnb115-in-parkinsons-disease-drosophila-line/