Session Time: 1:15pm-2:45pm
Location: Hall 3FG
Objective: We investigated the long-term consequences of sleep deprivation on short-term memory and emotion using a rotenone treated zebrafish model of Parkinson disease.
Background: Parkinson disease (PD) is the second most common neurodegenerative disorder in the world. Except for motor deficits, daily life of the patients is seriously affected by disturbances, cognitive impairment and emotional disorders. However, the effects of bad life habits such as stay up late on the patient’s cognition and mood are not clear.
Methods: Wild-type male zebrafish aged at 4 months were treated with rotenone (2g/L) for 4 weeks. Then rotenone treated zebrafish and the control fish were sleep deprived for 24 h. We evaluated the influence of sleep deprivation on motor function, short-term memory and emotional changes through locomotor activity, object discrimination task and light-dark box respectively. The effect of rotenone was verified through Western Blot. Dopaminergic systems were assessed using HPLC and Q-PCR.
Results: Our locomotor activity test showed no obvious difference between rotenone treated and control fish in the duration of freezing and swimming activity at a slow speed. However, the rotenone treated fish showed a reduction in swimming duration and distance traveled at a fast speed compared with control fish. But sleep deprivation had no effect on activity ability. Meanwhile, we found that TH expression reduced by 30% in rotenone treated fish. The object discrimination task exhibited that the short-term cognitive deficits of rotenone treated fish are more serious than the control fish after sleep deprivation. Light-dark box test showed that rotenone treated fish are more dysphoric than the control fish after sleep deprivation. Dopamine and DOPAC significantly reduced in rotenone treated fish compared with the control fish. However, the content of DOPAC recovered after sleep deprivation. The expression of DRD 2a, DRD 2b, DRD 2c and DRD 3 in rotenone treated zebrafish elevated compared with control group and sleep deprivation group. However, the rotenone treated zebrafish manifested a decrease level after sleep deprivation. DRD 1a and DRD 1b did not show any significant changes among the four groups.
Conclusions: Our findings suggest that after sleep deprivation, rotenone treated zebrafish may have a more severe damage on memory and emotional function, which may be related to the changes in the DA systems.
To cite this abstract in AMA style:Y. Xiong, D. Lv, S. Wei, F. Wang, C. Liu. The Effect of Sleep Deprivation on Short-Term Memory and Emotion of Rotenone Treated Zebrafish Model of Parkinson Disease [abstract]. Mov Disord. 2018; 33 (suppl 2). https://www.mdsabstracts.org/abstract/the-effect-of-sleep-deprivation-on-short-term-memory-and-emotion-of-rotenone-treated-zebrafish-model-of-parkinson-disease/. Accessed November 29, 2023.
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MDS Abstracts - https://www.mdsabstracts.org/abstract/the-effect-of-sleep-deprivation-on-short-term-memory-and-emotion-of-rotenone-treated-zebrafish-model-of-parkinson-disease/